Antibiotic Stock Solutions

Antibiotics Stock Solutions (Practically performed working concentration stock solutions for Agrobacterium and E.coli))
Kanamycin (stock 100 mg/ml)

1. Kanamycin-sulphate (1000 mg/10 ml)
2. Double distilled water up to 10 ml volume.
Double distilled de-ionized water was used for stock and final stock was filter sterilized using millipore filters of 0.22µm and stored at –200C in aliquots.

Cefotaxime (Claforan) (stock 100 mg/ml)

1. Cefotaxime (1000 mg/10 ml)
2. Double distilled water up to 10 ml volume.
Double distilled de-ionized water was used for stock and final stock was filter sterilized using millipore filters of 0.22µm and stored at –200C in aliquots.

Ampicilin (stock 100 mg/ml)

1. Ampicilin (1000 mg/10 ml)
2. Double distilled water up to 10 ml volume.
Double distilled de-ionized water was used for stock and final stock was filter sterilized using millipore filters of 0.22µm and stored at –200C in aliquots.